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Mouse fibroblast cell line survival during and after printing was favored by the presence of PG collagen as exhibited by the biological performance of the hydrogels. The addition of Ca 2+ ions into the system did present a synergistic effect: by one side, the in situ mineralization of the collagen occurred, and at same time, they were also useful to ionically cross-link the blends with alginate, avoiding the addition of any cytotoxic chemical cross-linking agent. In the second part, stable bioinks combining the biomimetically mineralized collagen with alginate (AG) (1:1, 1:2, 1:3, and AG) solution were used for 3D printing of hydrogels.
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Since then, sharks have diversified into over 500 species. 3 Acanthodians are often referred to as 'spiny sharks' though they are not part of Chondrichthyes proper, they are a paraphyletic assemblage leading to cartilaginous fish as a whole. These gorgeous stickers feature ferocious sharks in shades of gray and blue. The hydroxyapatite formation was confirmed by FT-IR, XRD, and TEM techniques. Under this broader definition, the earliest known sharks date back to more than 420 million years ago. Give your crafts a boost of three dimensional fun with Sharks 3D Stickers. In the first part, several parameters for collagen mineralization were tested until optimization. Only a limit of letter choice can be made each turn, so good luck. Choose the last letter of your word carefully since it also is the start letter of the next word. Type in 4 letter words as fast as possible to stay away from those dangerous jaws. In the present study, for the first time, the in situ mineralization (ideally hydroxyapatite formation) of blue shark ( Prionace glauca (PG)) collagen to fabricate 3D printable cell-laden hydrogels is proposed. Save the human from hungry shark apocalypse using your wisdom. Mineralization processes based on coprecipitation methods have been applied as a promising alternative to the most commonly used methods of polymer-ceramic combination, direct mixing, and incubation in simulated body fluid (SBF) or modified SBF.